Automated Trichloroacetic Acid Precipitation Method for Urine Total Protein
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چکیده
منابع مشابه
Interference by hemolysis with various methods for total calcium and its correction by trichloroacetic acid precipitation.
We evaluated the influence of hemolysis on total serum calcium as determined with the Du Pont aca, SMA 12/60, Ektachem 400, Corning 940 EGTA titrator, and the Beckman Astra 8, comparing results with those obtained by atomic absorption spectroscopy. We find that hemoglobin does not influence calcium measurements with the SMA 12/60, Ektachem 400, or Beckman Astra 8. The presence of hemoglobin exc...
متن کاملAlpha 1-acid glycoprotein decreases recovery of total protein in urine when trichloroacetic acid is used to precipitate the proteins.
Total urine protein was measured in 132 samples by an automated benzethonium chloride method and the Ponceau-S/trichloroacetic acid (PS/TCA) method. Of these, 27% gave a result 0.1 g/L or more higher by the benzethonium chloride method. Of this 27%, most contained an abnormally high concentration of the acute-phase reactant, alpha 1-acid glycoprotein. By assaying urine containing added alpha 1-...
متن کاملTrichloroethanol and Trichloroacetic Acid in Urine
When trichloroacetic acid (TCA) and trichloroethanol (TCE) in the urine of human subjects, rabbits, rats, and guinea-pigs were determined by Seto and Schultze's (I956) method, the recovery of TCE, added to urine, varied widely from 8i 3% in guinea-pig urine to i6-2% in rat urine. To obtain the maximum recovery of added TCE, Seto and Schultze's method was further modified to give satisfactory re...
متن کاملDirect analysis for urinary protein with biuret reagent, with use of urine ultrafiltrate blanking: comparison with a manual biuret method involving trichloroacetic acid precipitation.
We describe a method for measuring urinary protein with a centrifugal analyzer. Biuret reagent is used, and blanking with an ultrafiltrate of urine eliminates interferences from the nonprotein, biuret-positive chromogens in urine. We compare results by this new method with those by a manual method in which trichloroacetic acid precipitation and biuret reagent are used. The new method shows good...
متن کاملOptimal Concentration of 2,2,2-Trichloroacetic Acid for Protein Precipitation Based on Response Surface Methodology.
For low protein concentrations containing biological samples (in proteomics) and for non proteinaceous compound assays (in bioanalysis), there is a critical need for a simple, fast, and cost-effective protein enrichment or precipitation method. However, 2,2,2-trichloroacetic acid (TCA) is traditionally used for protein precipitation at ineffective concentrations for very low protein containing ...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 1987
ISSN: 0004-5632,1758-1001
DOI: 10.1177/000456328702400203